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| Figure 1. The Hybrid Capture System is a signal amplification assay that uses antibody capture and signal detection. Specimens are combined with a basic solution that disrupts the virus and releases target DNA. The DNA then combines with RNA probes, creating a DNA–RNA hybrid. Hybrids are captured onto a solid phase that has been coated with antibodies specific for the hybrids. Captured hybrids are detected with antibodies bound to alkaline phosphatase. Upon release by alkaline phosphatase, a dioxetane substrate produces chemiluminescence that is measured with a luminometer. |
